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[分享] 植物代谢组学案例分析

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发表于 2017-3-17 10:46:19 | 显示全部楼层 |阅读模式

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植物组学:大豆幼苗叶片对盐胁迫的响应 研究对象:大豆分析检测平台:GC-TOF/MS (BIOTREE)期刊:PLoS ONE影响因子:3.057发表时间:2016 摘要:Clarification of the metabolic mechanisms underlying salt stress responses in plants will allow further optimization of crop breeding and cultivation to obtain high yields in salinealkali land. Here, we characterized 68 differential metabolites of cultivated soybean (Glycine max) and wild soybean (Glycine soja) under neutral-salt and alkali-salt stresses using gas chromatography-mass spectrometry (GC-MS)-based metabolomics, to reveal the physiologicaland molecular differences in salt tolerance. According to comparisons of growth parameters under the two kinds of salt stresses, the level of inhibition in wild soybean was lower than in cultivated soybean, especially under alkali-salt stress. Moreover, wild soybean contained significantly higher amounts of phenylalanine, asparagine, citraconic acid, citramalic acid, citric acid and α-ketoglutaric acid under neutral-salt stress, and higher amounts of palmitic acid, lignoceric acid, glucose, citric acid and α-ketoglutaric acid under alkali-salt stress, than cultivated soybean. Further investigations demonstrated that the ability of wild soybean to salt tolerance was mainly based on the synthesis of organic and amino acids, and the more active tricarboxylic acid cycle under neutral-salt stress. In addition, the metabolite profiling analysis suggested that the energy generation from β-oxidation, glycolysis and the citric acid cycle plays important roles under alkali-salt stress. Our results extend the understanding of mechanisms involved in wild soybean salt tolerance and provide an important reference for increasing yields and developing salt-tolerant soybean cultivars. 研究背景:大豆是重要的重要的经济和油料作物,然而培育过程中其耐盐能力逐渐下降,严重影响植株生长和产品质量。野生大豆与培育大豆亲缘关系紧密,但常表现出更强的耐盐能力。通过对野生株研究,Na+、抗氧化酶和次级代谢途径等在植株不同部位的表达均对耐盐过程有影响。在应激过程中,野生株中有更高的氨基酸、二糖类物质积累,而不饱和脂肪酸、羧酸和单糖类物质低于培育植物。代谢物组学可更全面揭示植株对环境的响应过程,包括植物机能、代谢网络、代谢调控机制和表型等均可通过对代谢物的考察进行研究。此前代谢组学这一研究工具在植物应激过程的分析研究中已得到广泛使用。本文尝试从耐受能力不同的植株出发,尝试通过比较发现大豆耐盐能力相关代谢途径并为后续代谢工程改造提供基础。
5 p7 C0 \+ i+ ]阅读文献下载地址:[url]http://book.yunzhan365.com/jtuc/wglu/index.htmlZhang[/url] J, Yang D, Li M, et al. Metabolic Profiles Reveal Changes in Wild and Cultivated Soybean Seedling Leaves under Salt Stress:[J]. Plos One, 2016, 11(7).
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